Course syllabus

Biokemi
Biochemistry

KBKA10, 7,5 credits, G1 (First Cycle)

Valid for: 2019/20
Decided by: PLED B/K
Date of Decision: 2019-03-29

General Information

Main field: Technology.
Compulsory for: B2
Language of instruction: The course will be given in English

Aim

The aim of the course is to give basic knowledge in biochemistry, particularly within protein chemistry, enzymology, photosynthesis, and metabolism as well as basic abilities in biochemical laboratory work.

Learning outcomes

Knowledge and understanding
For a passing grade the student must

• be able to explain and describe central methods for purification and characterization of proteins
• be able to explain and describe enzyme function and regulation
• be able to discuss and describe the three steps of energy metabolism on an semi-advanced level
• be able to use the concept of oxidation/reduction to explain cellular energy transformations, including photosynthesis
• be able to describe the cellular synthesis of biomolecules on a basic level
• be able to explain, describe and generalise central concepts of metabolic regulation

Competences and skills
For a passing grade the student must

• be able to in a group design and carry out a protein purification process in laboratory scale
• have the ability to master some common biochemical laboratory techniques

Judgement and approach
For a passing grade the student must

 

• be able to to estimate the feasibility of a method for purifying a protein from a biological material.
• have the ability to make an overall assessment of the nutritional contents in a food product and how this can influence the health status of an individual.

Contents

• different methods for protein purification and protein characterization
• examples of specific enzyme mechanisms
• carbohydrates in biological systems
• the three steps of energy metabolism
• control of metabolism by different methods
• mechanisms of hormone action
• electron transport and its relation to ATP synthesis and photosynthesis
• biosynthesis of central biomolecules
• purification of an enzyme in a laboratory practical

Examination details

Grading scale: TH - (U,3,4,5) - (Fail, Three, Four, Five)
Assessment: Written examination and a report from a laboratory practical.

The examiner, in consultation with Disability Support Services, may deviate from the regular form of examination in order to provide a permanently disabled student with a form of examination equivalent to that of a student without a disability.

Parts
Code: 0117. Name: Biochemistry, Theory.
Credits: 6. Grading scale: TH. Assessment: Written examination. Contents: Different methods for protein purification and protein characterization. Examples of specific enzyme mechanisms. Carbohydrates in biological systems. The three steps of energy metabolism. Control of metabolism by different methods. Mechanisms of hormone action. Electron transport and its relation to ATP synthesis and photosynthesis. Biosynthesis of central biomolecules.
Code: 0217. Name: Biochemistry, Practicals.
Credits: 1,5. Grading scale: UG. Assessment: Report on practicals. Contents: The laboratory practical covers the purification of genetically modified lactate dehydrogenase from E.coli.

Admission

The number of participants is limited to: No
The course overlaps following course/s: KBK010, KBK020, KBK011

Reading list

• Berg, J.M., Tymoczko, J.L., Gatto, G.J. and Stryer, L.: Biochemistry. W.H. Freeman & Co, 2015, ISBN: 978-1-4641-2610-9.

Contact and other information

Course coordinator: Nélida Leiva Eriksson , nelida_leiva.eriksson@tbiokem.lth.se
Course homepage: http://www.tbiokem.lth.se/english/education/biochemistry/