Course syllabus

Biokemi
Biochemistry

KBK011, 7,5 credits, G1 (First Cycle)

Valid for: 2012/13
Decided by: Education Board 2
Date of Decision: 2012-04-04

General Information

Main field: Technology.
Compulsory for: B2
Language of instruction: The course will be given in Swedish

Aim

The aim of the course is to give basic knowledge in biochemistry, particularly within protein chemistry, enzymology and metabolism as well as basic abilities in biochemical laboratory work.

Learning outcomes

Knowledge and understanding
For a passing grade the student must

• be able to explain and describe central methods for purification and characterization of proteins
• be able to explain and describe enzyme function and regulation
• be able to discuss and describe the three steps of energy metabolism on an semi-advanced level
• be able to use the concept of oxidation/reduction to explain cellular energy transformations
• be able to describe the cellular synthesis of biomolecules on a basic level
• be able to explain, describe and generalise central concepts of metabolic regulation

Competences and skills
For a passing grade the student must

• be able to in a group design and carry out a protein purification process in laboratory scale
• have the ability to master some common biochemical laboratory techniques

Contents

• different methods for protein purification and protein characterization
• examples of specific enzyme mechanisms
• carbohydrates in biological systems
• the three steps of energy metabolism
• control of metabolism by different methods
• mechanisms of hormone action
• electron transport and its relation to ATP synthesis
• biosynthesis of central biomolecules
• purification of an enzyme in a laboratory practical

Examination details

Grading scale: TH
Assessment: Written examination and a passed report from a laboratory practical.

Parts
Code: 0105. Name: Biochemistry, Theory.
Credits: 6. Grading scale: TH. Assessment: Written examination. Contents: Different methods for protein purification and protein characterization. Examples of specific enzyme mechanisms. Carbohydrates in biological systems. The three steps of energy metabolism. Control of metabolism by different methods. Mechanisms of hormone action. Electron transport and its relation to ATP synthesis. Biosynthesis of central biomolecules.
Code: 0205. Name: Biochemistry, Practicals.
Credits: 1,5. Grading scale: UG. Assessment: Passed report. Contents: The laboratory practical covers the purification of genetically modified lactate dehydrogenase from E.coli.

Admission

The number of participants is limited to: No
The course overlaps following course/s: KBK010, KBK020

Reading list

• Berg, J.M, Tymoczko, J.L and Stryer, L.: Biochemistry, W.H. Freeman & Co, San Fransisco 2007, ISBN:0-7167-8724-5.

Contact and other information

Course coordinator: Professor Leif Bülow, Leif.Bulow@tbiokem.lth.se
Course homepage: http://www.tbiokem.lth.se/homepage/kursen/Biokemi(BB).html