Course syllabus

Biofysikalisk kemi
Biophysical Chemistry

KFKN10, 7,5 credits, A (Second Cycle)

Valid for: 2020/21
Decided by: PLED B/K
Date of Decision: 2020-03-27

General Information

Main field: Biotechnology.
Elective Compulsory for: MLAK1
Elective for: B4-l, B4-mb, K4-l, N4
Language of instruction: The course will be given in English on demand


The course aims at giving the student:

Learning outcomes

Knowledge and understanding
For a passing grade the student must

Competences and skills
For a passing grade the student must

Judgement and approach
For a passing grade the student must


The course addresses the following main topics:

The chemical building-blocks and three-dimensional structures of proteins: Structure analysis by X-ray crystallography; Structure and sequence databases; Bioinformatics.

Protein characterization by optical spectroscopy: Physical principles and applications of fluorescence and circular dichroism spectroscopy.

Polypeptide conformation: Models of polymer conformation and conformational transitions; Conformational entropy; Folding cooperativity.

Protein energetics and stability: Packing; Hydration; Electrostatics; Thermal and solvent-induced denaturation; Differential scanning calorimetry.

Protein dynamics: Kinetic models; Proton exchange; Diffusion control; Protein folding; Computer simulation of proteins.

Nuclear magnetic resonance: Principles of NMR spectroscopy and relaxation; Analysis of structure, interactions and dynamics of proteins in solution.

Association processes: Ligand binding; Allostery; Protein aggregation; Isothermal titration calorimetry; Surface plasmon resonance.

Examination details

Grading scale: TH - (U,3,4,5) - (Fail, Three, Four, Five)
Assessment: The final grade is based on weekly take-home problem assignments (50%) and a written midterm exam (50%). Approved laboratory practicals and oral presentation are needed for a passing grade. If necessary, reexamination (of the midterm exam) will be performed orally.

The examiner, in consultation with Disability Support Services, may deviate from the regular form of examination in order to provide a permanently disabled student with a form of examination equivalent to that of a student without a disability.


Admission requirements:

Assumed prior knowledge: KFKF01 Molecular Driving Forces 2: Interactions and Dynamics
The number of participants is limited to: No
The course overlaps following course/s: KFK032

Reading list

Contact and other information

Course coordinator: Kristofer Modig,
Course homepage:
Further information: The course emphasizes active processing of knowledge through take-home problem assignments and laboratory practicals. Five lectures are devoted to research in protein science, where the students present and critically discuss recent research results.