Course syllabus

Biofysikalisk kemi
Biophysical Chemistry

KFK032, 7,5 credits, A (Second Cycle)

Valid for: 2016/17
Decided by: Education Board C
Date of Decision: 2016-04-12

General Information

Main field: Biotechnology.
Elective for: B4-l, B4-mb, K4-l
Language of instruction: The course will be given in English on demand


The course aims at giving the student: 

Learning outcomes

Knowledge and understanding
For a passing grade the student must

Competences and skills
For a passing grade the student must

Judgement and approach
For a passing grade the student must


The course addresses the following main topics:

The chemical building-blocks and three-dimensional structures of proteins: Structure analysis by X-ray crystallography; Structure and sequence databases; Bioinformatics.

Protein characterization by optical spectroscopy: Physical principles and applications of fluorescence and circular dichroism spectroscopy.

Polypeptide conformation: Models of polymer conformation and conformational transitions; Conformational entropy; Folding cooperativity.

Protein energetics and stability: Packing; Hydration; Electrostatics; Thermal and solvent-induced denaturation; Differential scanning calorimetry.

Protein dynamics: Kinetic models; Proton exchange; Diffusion control; Protein folding; Computer simulation of proteins.

Nuclear magnetic resonance: Principles of NMR spectroscopy and relaxation; Analysis of structure, interactions and dynamics of proteins in solution.

Association processes: Ligand binding; Allostery; Protein aggregation; Isothermal titration calorimetry; Surface plasmon resonance.

Examination details

Grading scale: TH
Assessment: The final grade is based on weekly take-home problem assignments (50%) and a written midterm exam (50%). Laboratory practicals and oral presentation must be completed. If necessary, reexamination (of the midterm exam) will be performed orally.


Admission requirements:

The number of participants is limited to: No

Reading list

Contact and other information

Course coordinator: prof Bertil Halle,
Course homepage:
Further information: The course emphasizes active processing of knowledge through take-home problem assignments and laboratory practicals. Five lectures are devoted to research in protein science, where the students present and critically discuss recent research results.