Course syllabus

Projektkurs i kromatografisk analys
Project in Chromatographic Analysis

KAKN05, 15 credits, A (Second Cycle)

Valid for: 2012/13
Decided by: Education Board 2
Date of Decision: 2012-04-04

General Information

Main field: Biotechnology.
Elective Compulsory for: MBIO1, MLIV1
Elective for: B4-l, B4-mb, B4-lm, K4-l
Language of instruction: The course will be given in English on demand

Aim

The aim of the course is to provide advanced insights into and skills in analytical separation techniques that are commonly used in pharmacetutical, food, environmental, polymer, biotechnical and biochemical analysis.

Learning outcomes

Knowledge and understanding
For a passing grade the student must

• be able to explain advanced theories in different analytical principles and techniques comprised by the course
• be able to describe, evaluate the choice of and optimize the chosen separation technique for analysis of low and high molecular weight compounds
• be able to understand the importance of adequate sample preparation prior to instrumental analysis
• be able to explain the meaning of concepts used in quality assessment of analytical results
• understand how a project group including knowledge on some common tools for project management

Competences and skills
For a passing grade the student must

• show abilities in performing advanced analysis (qualitative and quantitative) of for example pharmaceuticals in biological samples (blood and/or urine) and/or proteins
• be able to tackle an analytical project by critical evaluation of scientific reports and from these be able to construct and test a method for practical analysis
• be able to perform quality assessment and report analytical results both orally and in writing
• be able to search, evaluate and use information relevant for the project using university library resources and open electronic sources
• be able to reflect on the role that the student will take in a project group

Judgement and approach
For a passing grade the student must

• understand how the choice of an analytical technique and method affects the quality of the analytical result
• for a given problem be able to choose an optimal analytical method

Contents

The course comprises different analytical separation techniques and methods, such as HPLC and LC/MS, GC and GC/MS, CE (capillary electrophoresis), CEC (capillary electrochromatography), microfluidics and FFF (field flow fractionation), for characterisation and quantitation of low and high molecular weight substances normally utilized in pharmaceutical, food, environmental, biotechnical and biochemical analysis.

An important part of the course is spent on strategies for sample preparation and optimization of separation and detection conditions. Also the importance of method validation and quality assessment is highlighted.

The second part of the course is spent on planning and performing analysis relevant to industry and/or research. Important concepts regarding project management will be supported through lectures and seminars.

Examination details

Grading scale: TH
Assessment: Written examination covering lectures, course literature and the experimental parts of the course. To receive a passing grade for the course, students must have also satisfactorily completed the required laboratory work and project.

Parts
Code: 0111. Name: Written Examination.
Credits: 15. Grading scale: TH.
Code: 0211. Name: Laboratory Experiments.
Credits: 0. Grading scale: UG.
Code: 0311. Name: Project.
Credits: 0. Grading scale: UG.

Admission

Admission requirements:

• KAKF01

The number of participants is limited to: No
The course overlaps following course/s: KAK050, KAK070

Reading list

• Harris, D C: Quantitative Chemical Analysis. 8th ed. Freeman 2010.
• ISBN: 1-4292-3989-1.
• Compendia.

Contact and other information

Course coordinator: universitetslektor Margareta Sandahl, Margareta.Sandahl@organic.lu.se
Course homepage: http://www.kilu.lu.se/cas/education/undergraduate_education/kemibioteknik/kakn05/