Syllabus academic year 2009/2010
(Created 2009-08-11.)

Higher education credits: 7,5. Grading scale: TH. Level: A (Second level). Language of instruction: The course will be given in English on demand. Alternative for: MBIO1, MLIV1. Optional for: B4l, B4mb, K4l. Course coordinator: Prof. Staffan Nilsson, och Senior lecturer Margareta Sandahl,, Teknisk analytisk kemi. Prerequisites: KAK050 Chromatographic Analysis. The course might be cancelled if the numer of applicants is less than 6. The number of participants is limited to 18 Selection criteria: Credits remaining for the degree. Assessment: Written examination covering lectures, course literature and the experimental parts of the course. For a passing grade, also passed laboratory work, reports and taking an active part in the down scaling exercise are required. Home page:

The aim of the course is to give advanced knowledge about the technical use of and possibility to create new analytical-chemical methods for characterisation and quantitation of low and high molecular weight compounds for drug-, food-, environmental-, polymer-, analytical-, biotechnology- and biochemical- analysis.

Knowledge and understanding
For a passing grade the student must

Skills and abilities
For a passing grade the student must

Judgement and approach
For a passing grade the student must

Sample pre-treatment, especially for bioanalysis.

Microanalytical techniques: in liquid chromatography (LC) and capillary electrophoresis (CE) and through microfabrication in silicon and plastic chips ("Lab-on-a-chip").

Macromolecule analysis: The special demands and methods in analysis of proteins within molecular biology are illustrated with e.g. drug proteins as examples. This represents the experimental basis for proteom analysis. Possibilities of polysaccharide analysis in modified natural sources are enlightened. Mass spectrometry (MS) with emphasis on LC-MS with electrospray ionisation (ESI) and matrix assisted laser desorption –ionisation- time of flight mass spectrometry (MALDI-TOFMS) is treated.

Application areas: Drug analysis: particularly enantiomer analysis, product analysis with emphasis on stability testing and dissolution profiles of drug formulations, and bioanalysis such as methods in pharmacokinetic studies. Chromatography - mass spectrometry is treated. Analytical biotechnology: protein characterisation and peptide analysis using LC, CE, and MS, particularly regarding recombinant proteins and drug peptides. Emphasis on size exclusion chromatography (SEC) and field-flow fractionation (FFF) in analysis of protein aggregation and reversed-phase chromatography (RPLC), ion-exchange chromatography (IEC), and hydrophobic interaction chromatography (HIC) in peptide and protein identification and characterisation, also in connection with peptide mapping after trypsin degradation and amino acid sequencing. The use of MALDI-TOF MS and LC-ESI MS in peptide and protein analysis is discussed.

Polymer analysis: SEC and FFF of modified natural polymers.

Detection systems: Electrochemical, optical (UV-spectrophotometry, fluorimetry, light scattering), and mass spectrometric detectors.

Compendia. Div. of Analytical Chemistry.
Laboratory text. Div. Analytical Chemistry.
Harris, D C: Quantitative Chemical Analysis. Seventh ed. Freeman 2007. ISBN: 0-7167-7041-5.