Course syllabus

Kromatografisk analys
Chromatographic Analysis

KASN05, 7,5 credits, A (Second Cycle)

Valid for: 2020/21
Decided by: PLED B/K
Date of Decision: 2020-03-27

General Information

Language of instruction: The course will be given in English on demand

Aim

The aim of the course is to provide advanced knowledge in and insight into modern analytical techniques including chromatography, capillary electrophoresis, field-flow fractionation and mass spectrometry.

Learning outcomes

Knowledge and understanding
For a passing grade the student must

• be able to explain advanced theories in different analytical principles and techniques comprised by the course
• be able to describe, evaluate the choice of and optimize the chosen separation technique for analysis of low and high molecular weight compounds
• be able to explain the meaning of concepts used in quality assessment of analytical results

Competences and skills
For a passing grade the student must

• show abilities in handling advanced analytical techniques
• be able to interpret experimentally generated data and compile these into concise, clearly written reports

Judgement and approach
For a passing grade the student must

• understand how the choice of an analytical technique and method affects the quality of the analytical result

Contents

The course comprises different analytical separation techniques and methods, such as HPLC, UHPLC and LC/MS, GC and GC/MS, CE (capillary electrophoresis), SFC (supercritical fluid chromatography) and FFF (flow field fractionation), for characterisation and quantitation of low and high molecular weight substances normally utilized in pharmaceutical, food, environmental, biotechnical and biochemical analysis.

An important part of the course is spent to strategies for optimization of separation and detection conditions. Also the importance of method validation and quality assessment is highlighted.

Examination details

Grading scale: TH - (U,3,4,5) - (Fail, Three, Four, Five)
Assessment: Written examination, laboratory work and reports. The final grade will be based on the written examination.

The examiner, in consultation with Disability Support Services, may deviate from the regular form of examination in order to provide a permanently disabled student with a form of examination equivalent to that of a student without a disability.

Parts
Code: 0117. Name: Written Examination.
Credits: 7,5. Grading scale: TH. Assessment: Written examination covering lectures, course literature and the experimental parts of the course.
Code: 0217. Name: Laboratory Experiments.
Credits: 0. Grading scale: UG. Assessment: Mandatory attendance at laboratory exercises and approved written reports.

Admission

Assumed prior knowledge: KAKF01 Analytical Chemistry
The number of participants is limited to: 24
Selection: Completed university credits within the programme. Priority is given to students enrolled on programmes that include the course in their curriculum.
The course overlaps following course/s: KAKN05, KAK050

Reading list

• Harris, D. C.: Quantitative Chemical Analysis. 9th ed. Freeman , 2015, ISBN: 1-4641-3538-X.
• Compendia.

Contact and other information

Course coordinator: Universitetslektor Margareta Sandahl, margareta.sandahl@chem.lu.se
Course homepage: http://www.kilu.lu.se/cas/education/undergraduate_education/kemibioteknik/kasn05/