Course syllabus

Kromatografisk analys
Chromatographic Analysis

KAK050, 7,5 credits, A (Second Cycle)

Valid for: 2015/16
Decided by: Education Board C
Date of Decision: 2015-04-20

General Information

Elective Compulsory for: MBIO1
Elective for: B4-l, B4-mb, B4-lm, K4-l, MLIV1
Language of instruction: The course will be given in English on demand

Aim

The aim of the course is to provide advanced knowledge in and insight into analytical separation techniques.

Learning outcomes

Knowledge and understanding
For a passing grade the student must

• be able to explain advanced theories in different analytical principles and techniques comprised by the course
• be able to describe, evaluate the choice of and optimize the chosen separation technique for analysis of low and high molecular weight compounds
• be able to understand the importance of adequate sample preparation prior to instrumental analysis
• be able to explain the meaning of concepts used in quality assessment of analytical results

Competences and skills
For a passing grade the student must

• show abilities in handling advanced analytical techniques
• be able to interpret experimentally generated data and compile these into concise, clearly written technical reports

Judgement and approach
For a passing grade the student must

• understand how the choice of an analytical technique and method affects the quality of the analytical result

Contents

The course comprises different analytical separation techniques and methods, such as HPLC and LC/MS, GC and GC/MS, CE (capillary electrophoresis), and FFF (flow field fractionation), for characterisation and quantitation of low and high molecular weight substances normally utilized in pharmaceutical, food, environmental, biotechnical and biochemical analysis.

An important part of the course is spent to strategies for optimization of separation and detection conditions. Also the importance of method validation and quality assessment is highlighted.

Examination details

Grading scale: TH
Assessment: Written examination covering lectures, course literature and the experimental parts of the course. To receive a passing grade for the course, students must have also satisfactorily completed the required laboratory work and reports.

Parts
Code: 0110. Name: Written Examination.
Credits: 7,5. Grading scale: TH.
Code: 0210. Name: Laboratory Experiments.
Credits: 0. Grading scale: UG.

Admission

Required prior knowledge: KAKF01
The number of participants is limited to: 24
Selection: Credits remaining for the degree.
The course overlaps following course/s: KAKN05

Reading list

• Harris, D. C.: Quantitative Chemical Analysis. 8th ed. Freeman , 2010, ISBN: 1-4292-3989-1.
• Compendia.

Contact and other information

Course coordinator: Universitetslektor Margareta Sandahl, margareta.sandahl@chem.lu.se
Course homepage: http://www.kilu.lu.se/cas/education/undergraduate_education/kemibioteknik/kak050/