Course syllabus

Biotekniska separationsprocesser
Separations in Biotechnology

KBT060, 7,5 credits, G2 (First Cycle)

Valid for: 2013/14
Decided by: Education Board C
Date of Decision: 2013-04-15

General Information

Main field: Biotechnology.
Compulsory for: B4-pt
Elective Compulsory for: MBIO1
Elective for: B4-mb, K4-p
Language of instruction: The course will be given in English


The objective of the course is to provide a general overview of bioseparation processes starting from a raw material and all the way through to a final product giving basic knowledge of physico-chemical principles behind different methods of separation of biomolecules. An efficient bioseparation process defines often the market life of the product as purification expenses comprise more than 70 % of the final product cost. Applying and integrating knowledge previously gained by students, the course will provide a deeper understanding of basic principles of bioseparation processes and the factors affecting the performance of the individual separation operations. The course will illustrate the challenges for biotechnologists using the examples of existing processes.

Learning outcomes

Knowledge and understanding
For a passing grade the student must

Competences and skills
For a passing grade the student must


Critical analysis of different separation methods emphasizing the differences between purification of small and large molecules. Cell disintegration, membrane filtration, precipitation, extraction and chromatography of biomolecules as well as product formulation and process monitoring are discussed. The course involves also a laboration on modern protein chromatography. An important part of the course is the presentation of the case study.

Examination details

Grading scale: TH
Assessment: Written exam, approved labreport and presentation of the case study.

Code: 0108. Name: Separations in Biotechnology.
Credits: 6. Grading scale: TH. Assessment: Written exam.
Code: 0208. Name: Laboratory Work.
Credits: 1,5. Grading scale: UG. Assessment: Approved written report.


Required prior knowledge: KBK011 Biochemistry, KETF010 Separation Processes.
The number of participants is limited to: No

Reading list

Contact and other information

Course coordinator: Gashaw Mamo,
Course homepage: