Syllabus academic year 2011/2012
(Created 2011-09-01.)
SEPARATIONS IN BIOTECHNOLOGYKBT060
Credits: 7,5. Grading scale: TH. Cycle: G2 (First Cycle). Main field: Technology. Language of instruction: The course will be given in English. Compulsory for: B4pt. Alternative for: MBIO1. Optional for: B4mb, K4p. Course coordinator: Professor Olle Holst, Olle.Holst@biotek.lu.se, Biotechnology. Recommended prerequisits: KBK011 Biochemistry, KETF010 Separation Processes. The course might be cancelled if the number of applicants is less than 6. Assessment: Written exam, approved labreport and presentation of the case study. Parts: 2. Home page: http://www.biotek.lu.se/KBT060.

Aim
The objective of the course is to provide a general overview of bioseparation processes starting from a raw material and all the way through to a final product giving basic knowledge of physico-chemical principles behind different methods of separation of biomolecules. An efficient bioseparation process defines often the market life of the product as purification expenses comprise more than 70 % of the final product cost. Applying and integrating knowledge previously gained by students, the course will provide a deeper understanding of basic principles of bioseparation processes and the factors affecting the performance of the individual separation operations. The course will illustrate the challenges for biotechnologists using the examples of existing processes.

Knowledge and understanding
For a passing grade the student must

Skills and abilities
For a passing grade the student must

Contents
Critical analysis of different separation methods emphasizing the differences between purification of small and large molecules. Cell disintegration, membrane filtration, precipitation, extraction and chromatography of biomolecules as well as product formulation and process monitoring are discussed. The course involves also a laboration on modern protein chromatography. An important part of the course is the presentation of the case study.

Literature
Hatti-Kaul, R., Mattiasson, B., (2003) Isolation and Purification of Proteins, Marcel Dekker, NY. Handouts.
Schuler, M. L., Kargi, F. : Bioprocess Engineering - Basic Concepts. Prentice Hall, New Jersey. Chapter 11.

Parts

Code: 0108. Name: Separations in Biotechnology.
Higher education credits: 6. Grading scale: TH. Assessment: Written exam.

Code: 0208. Name: Laboratory Work.
Higher education credits: 1,5. Grading scale: UG. Assessment: Approved written report.