Syllabus 2011/2012 KBT050

Syllabus academic year 2011/2012
(Created 2011-09-01.)

BIO ANALYTICAL CHEMISTRY

KBT050

Credits: 7,5. Grading scale: TH. Cycle: G2 (First Cycle). Main field: Technology. Language of instruction: The course will be given in English. Alternative for: MBIO2, MLIV2. Optional for: B4mb, B4pt, N4. Course coordinator: Maria Andersson, Maria.Andersson@biotek.lu.se, Biotechnology. Prerequisites: KBK011 Biochemistry, advanced course. The course might be cancelled if the number of applicants is less than 6. Assessment: Written examination. Apart from approved laboratory exercises and assignment, at least 50 % of the given problems in the written exam must be solved correctly. Questions based mostly on general understanding not so much on details. Laborations will be judged on the basis of the laboration report and the Assignment will be judged based on written and oral presentation. Parts: 3. Home page: http://www.biotek.lu.se/kbt050.

Aim
The objective of the course in bioanalysis is to give an overview of the bioanalytical area and basic knowledge of the physico-chemical principles behind different bioanalytical methods. By applying and integrating knowledge previously gained by the student, the course will provide a deeper understanding of basic principles and factors that affect the choice of individual methods and steps.

Knowledge and understanding
For a passing grade the student must

understand the different bioanalytical principles that are part of the course. He or she should be able to describe these analytical principles and their strengths and weaknesses.
in addition, the student should understand the importance of retrieving samples in a correct way and to handle it in an adequate and reproducible manner.

Skills and abilities
For a passing grade the student must

faced with an analytical problem, be able to in a rational way, choose an adequate analytical method, well aware of its strengths and weaknesses.
through the laboratory exercises have gained certain experimental experience, for example concerning the handling of bioanalytical reagents.

Judgement and approach
For a passing grade the student must

- perform an assignment where she/he in a rational way and based on the theoretical part of the course should chose a method for solving a given analytical problem. The student will receive information about a actual analytical problem and present a suggestion of a solution using suitable analytical method/s.

- in a clear way present his/her experiences and conclusions in a written report and through an oral presentation.

- discuss and critically evaluate the methods/conclusion presented by other students.

Contents
This course covers the use of enzymes, antibodies, cells and DNA in analytical systems, e.g. biosensors. The course also covers important methods for analysis of biomolecules such as mass spectrometry and capillary electrophoresis as well as emerging technologies like lab-on-chip and microfluidic analytical systems. Analysis in well equipped laboratories as well as for field-adapted analysis is included. Applications in medical as well as food industry together with environmental monitoring is covered. Sampling and sample handling is considered.

Literature
File containing exclusively written material for the course and reprints

Parts

Code: 0111. Name: Assignment.
Higher education credits: 1. Grading scale: UG. Assessment: Approved written and oral presentation.

Code: 0211. Name: Bioanalysis.
Higher education credits: 5. Grading scale: TH. Assessment: Approved examination.

Code: 0311. Name: Laboratory Work.
Higher education credits: 1,5. Grading scale: UG. Assessment: Approved written report.