Syllabus 2011/2012 KAKN05

Syllabus academic year 2011/2012
(Created 2011-09-01.)

PROJECT IN CHROMATOGRAPHIC ANALYSIS

KAKN05

Credits: 15. Grading scale: TH. Cycle: A (Second Cycle). Main field: Technology. Language of instruction: The course will be given in English on demand. KAKN05 overlaps following cours/es: KAK050 and KAK070. Alternative for: MBIO1, MLIV1. Optional for: B4l, B4lm, B4mb, K4l. Course coordinator: Senior lecturer Margareta Sandahl, Margareta.Sandahl@organic.lu.se, Technical Analytical Chemistry. Prerequisites: KAKF01. Assessment: Written examination covering lectures, course literature and the experimental parts of the course. To receive a passing grade for the course, students must have also satisfactorily completed the required laboratory work and project. Home page: http://www.organic.lu.se.

Aim
The aim of the course is to provide advanced insights into and skills in analytical separation techniques that are commonly used in pharmacetutical, food, environmental, polymer, biotechnical and biochemical analysis.

Knowledge and understanding
For a passing grade the student must

be able to explain advanced theories in different analytical principles and techniques comprised by the course
be able to describe, evaluate the choice of and optimize the chosen separation technique for analysis of low and high molecular weight compounds
be able to understand the importance of adequate sample preparation prior to instrumental analysis
be able to explain the meaning of concepts used in quality assessment of analytical results
understand how a project group including knowledge on some common tools for project management

Skills and abilities
For a passing grade the student must

show abilities in performing advanced analysis (qualitative and quantitative) of for example pharmaceuticals in biological samples (blood and/or urine) and/or proteins
be able to tackle an analytical project by critical evaluation of scientific reports and from these be able to construct and test a method for practical analysis
be able to perform quality assessment and report analytical results both orally and in writing
be able to search, evaluate and use information relevant for the project using university library resources and open electronic sources
be able to reflect on the role that the student will take in a project group

Judgement and approach
For a passing grade the student must

understand how the choice of an analytical technique and method affects the quality of the analytical result
for a given problem be able to choose an optimal analytical method

Contents
The course comprises different analytical separation techniques and methods, such as HPLC and LC/MS, GC and GC/MS, CE (capillary electrophoresis), CEC (capillary electrochromatography), microfluidics and FFF (field flow fractionation), for characterisation and quantitation of low and high molecular weight substances normally utilized in pharmaceutical, food, environmental, biotechnical and biochemical analysis.

An important part of the course is spent on strategies for sample preparation and optimization of separation and detection conditions. Also the importance of method validation and quality assessment is highlighted.

The second part of the course is spent on planning and performing analysis relevant to industry and/or research. Important concepts regarding project management will be supported through lectures and seminars.

Literature
Harris, D C: Quantitative Chemical Analysis. 8th ed. Freeman 2010.
ISBN: 1-4292-3989-1
Compendia