Syllabus 2011/2012 KAK050

Syllabus academic year 2011/2012
(Created 2011-09-01.)

CHROMATOGRAPHIC ANALYSIS

KAK050

Credits: 7,5. Grading scale: TH. Cycle: A (Second Cycle). Main field: Technology. Language of instruction: The course will be given in English on demand. KAK050 overlaps following cours/es: KAKN05. Alternative for: MBIO1, MLIV1. Optional for: B4l, B4lm, B4mb, K4l. Course coordinator: Senior lecturer Margareta Sandahl, Margareta.Sandahl@organic.lu.se, Technical Analytical Chemistry. Prerequisites: KAKF01. The course might be cancelled if the number of applicants is less than 6. The number of participants is limited to 24 Selection criteria: Credits remaining for the degree. Assessment: Written examination covering lectures, course literature and the experimental parts of the course. To receive a passing grade for the course, students must have also satisfactorily completed the required laboratory work and reports. Home page: http://www.organic.lu.se.

Aim
The aim of the course is to provide advanced knowledge in and insight into analytical separation techniques.

Knowledge and understanding
For a passing grade the student must

be able to explain advanced theories in different analytical principles and techniques comprised by the course
be able to describe, evaluate the choice of and optimize the chosen separation technique for analysis of low and high molecular weight compounds
be able to understand the importance of adequate sample preparation prior to instrumental analysis
be able to explain the meaning of concepts used in quality assessment of analytical results

Skills and abilities
For a passing grade the student must

show abilities in handling advanced analytical techniques
be able to interpret experimentally generated data and compile these into concise, clearly written technical reports

Judgement and approach
For a passing grade the student must

understand how the choice of an analytical technique and method affects the quality of the analytical result

Contents
The course comprises different analytical separation techniques and methods, such as HPLC and LC/MS, GC and GC/MS, CE (capillary electrophoresis), CEC (capillary electrochromatography), microfluidis and FFF (flow field fractionation), for characterisation and quantitation of low and high molecular weight substances normally utilized in pharmaceutical, food, environmental, biotechnical and biochemical analysis.

An important part of the course is spent to strategies for optimization of separation and detection conditions. Also the importance of method validation and quality assessment is highlighted.

Literature
Harris, D C: Quantitative Chemical Analysis. 8th ed. Freeman 2010. ISBN: 1-4292-3989-1
Compendia