Syllabus academic year 2010/2011
(Created 2010-07-25.)
GENE TECHNOLOGYKBK041
Credits: 7,5. Grading scale: TH. Cycle: G2 (First Cycle). Main field: Technology. Language of instruction: The course might be given in English. Compulsory for: B3. Alternative for: MBIO1. Course coordinator: Professor Leif B├╝low, leif.bulow@tbiokem.lth.se and Dr. Maurizio Bettiga, Maurizio.Bettiga@tmb.lth.se, Pure and Applied Biochemistry. Prerequisites: KMB060 Mikrobiologi och KBK011 Biokemi. Assessment: Approved practicals, oral presentation of a literature task and written exam. Parts: 2. Further information: The course is given in collaboration between the divisions of Pure and Applied Biochemistry and Technical Microbiology. Home page: http://www.tbiokem.lth.se.

Aim
The aim of the course is to give the student the ability to understand and critically examine the possibilities and limitations in modern gene technology.

Knowledge and understanding
For a passing grade the student must

Skills and abilities
For a passing grade the student must

Judgement and approach
For a passing grade the student must

Contents
The course deals with the genetic and physiological prerequisites for gene modification of bacterial, yeast, plant and animal cells. Applications in chemical, food and pharmaceutical industries are emphasised. The laboratory part of the course includes the most frequently used methods in gene technology embracing isolation and mapping of DNA, PCR, vector construct, transformation and hybridisation.

Literature
Reece, R.J.: Analysis of Genes and Genomes, Wiley 2004. ISBN: 0470843802
Laboratory compendium

Parts

Code: 0105. Name: Gene Technology, Theory.
Higher education credits: 6. Grading scale: TH. Assessment: Written examination. Contents: The course deals with the genetic and physiological prerequisites for gene modification of bacterial, yeast, plant and animal cells. Applications in chemical, food and pharmaceutical industries are emphasised.

Code: 0205. Name: Gene Technology, Practicals.
Higher education credits: 1,5. Grading scale: UG. Assessment: Approved practicals. Contents: The laboratory part of the course includes the most frequently used methods in gene technology embracing isolation and mapping of DNA, PCR, vector construct, transformation and hybridisation.