Syllabus academic year 2010/2011
(Created 2010-07-25.)
ENZYME TECHNOLOGYKBK031
Credits: 7,5. Grading scale: TH. Cycle: A (Second Cycle). Main field: Technology. Language of instruction: The course will be given in English on demand. Alternative for: MBIO1, MLIV1. Optional for: B4mb, B4pt. Course coordinator: Professor Patrick Adlercreutz and Professor Per-Olof Larsson, per-olof.larsson@tbiokem.lth.se, Pure and Applied Biochemistry. Prerequisites: KBK011 Biochemistry. The course might be cancelled if the number of applicants is less than 8. The number of participants is limited to 32 Selection criteria: Credits awarded or credited within the study programme. Assessment: Written examination and passed reports from the laboratory practicals and passed literature discussion. Parts: 2. Home page: http://www.tbiokem.lth.se/Homepage/Kursen/ET/EThomepage.html.

Aim
The aim of the course is to give advanced knowledge about the technical use of enzymes and the possibilities to change and improve enzyme performance for adaptation to technical applications

Knowledge and understanding
For a passing grade the student must

Skills and abilities
For a passing grade the student must

Judgement and approach
For a passing grade the student must

Contents
The theoretical part of the course describes how the catalysts of the biological world, the enzymes, can be used in a variety of contexts. Examples are the use in bioanalysis, refining of food, washing powder, textile treatment and transformation of chemicals.

The general properties of enzymes are well known from previous courses. In this course is described how enzyme molecules can be modified in order to get improved properties for technical use. Examples include genetic and chemical modification and immobilization. Immobilization, i.e. the coupling of enzymes to carrier material, is treated thoroughly. Likewise are the kinetic properties of immobilized enzymes and ways to quantify these properties emphasized.

In the cell the enzymes function in an aqueous environment. Under certain circumstances enzymes can also work efficiently in a non-aqueous environment or in an environment with a low water activity. This opens up for unique possibilities for an enzymatic synthesis of a number of substances, including chiral substances. This kind of use of enzymes in non-conventional media is treated in great detail.

The laboratory practical has two parts. One part where basic techniques concerning immobilization of enzymes are investigated and one research related part where untested experiments with enzymes in organic solvents are designed and carried out.

Literature
Adlercreutz, P., Danielsson, B., Larsson, P-O., Månsson, M-O., Ramanathan, K.: Compendium: Enzyme Technology

Parts

Code: 0105. Name: Enzyme Technology, Theory.
Higher education credits: 4,5. Grading scale: TH. Assessment: Written examination and passed literature discussion. Contents: The course descibes how the catalysts of the biological world, the enzymes, can be used in a variety of contexts. Examples are the use in bioanalysis, refining of food, washing powder, textile treatment and transformation of chemicals. The general properties of enzymes are well known from previous courses. In this course is described how enzyme molecules can be modified in order to get improved properties for technical use. Examples include genetic and chemical modification and immobilization. Immobilization, i.e. the coupling of enzymes to carrier material, is treated thoroughly. Likewise are the kinetic properties of immobilized enzymes and ways to quantify these properties emphasized. In the cell the enzymes function in an aqueous environment. Under certain circumstances enzymes can also work efficiently in a non-aqueous environment or in an environment with a low water activity. This opens up for unique possibilities for an enzymatic synthesis of a number of substances, including chiral substances. This kind of use of enzymes in non-converntional media is treated in great detail.

Code: 0205. Name: Enzyme Technology, Practicals.
Higher education credits: 3. Grading scale: UG. Assessment: Passed written reports. Contents: The laboratory practical has two parts. One part where basic techniques concerning immobilization of enzymes are investigated and one reasearch related part where untested experiments with enzymes in organic solvents are designed and carried out.