Syllabus academic year 2010/2011
(Created 2010-07-25.)
PROJECT IN CHROMATOGRAPHIC ANALYSISKAKN05
Credits: 15. Grading scale: TH. Cycle: A (Second Cycle). Main field: Technology. Language of instruction: The course will be given in English on demand. KAKN05 overlaps following cours/es: KAK050 and KAK070. Alternative for: MBIO1, MLIV1. Optional for: B4l, B4lm, B4mb, K4l. Course coordinator: Senior lecturer Margareta Sandahl, Margareta.Sandahl@organic.lu.se and Clas Wesén, 0703-978762, Technical Analytical Chemistry. Prerequisites: KAKF01. Assessment: Written examination covering lectures, course literature and the experimental parts of the course. To receive a passing grade for the course, students must have also satisfactorily completed the required laboratory work and project. Home page: http://www.organic.lu.se.

Aim
The aim of the course is to provide advanced insights into and skills in analytical separation techniques that are commonly used in pharmacetutical, food, environmental, polymer, biotechnical and biochemical analysis.

Knowledge and understanding
For a passing grade the student must

Skills and abilities
For a passing grade the student must

Judgement and approach
For a passing grade the student must

Contents
The course comprises different analytical separation techniques and methods, such as HPLC and LC/MS, GC and GC/MS, CE (capillary electrophoresis) and CEC (capillary electrochromatography) and FFF (field flow fractionation), for characterisation and quantitation of low and high molecular weight substances normally utilized in pharmaceutical, food, environmental, biotechnical and biochemical analysis.

An important part of the course is spent on strategies for optimization of separation and detection conditions. Also the importance of method validation and quality assessment is highlighted.

The second part of the course is spent on planning and performing analysis relevant to industry and/or research.

Literature
Harris, D C: Quantitative Chemical Analysis. Seventh ed. Freeman 2007. ISBN: 0-7167-7041-5.
Compendia. Div. of Technical Analytical Chemistry.
Laboratory text. Div. of Technical Analytical Chemistry.