Syllabus 2009/2010 TEK287

Syllabus academic year 2009/2010
(Created 2009-08-11.)

BIOCHEMISTRY

TEK287

Higher education credits: 15. Grading scale: TH. Level: G2 (First level). Language of instruction: The course will be given in Swedish. TEK287 overlap following cours/es: MOB102, MOB102, MOB102 och MOB102. Optional for: N4, N4nbm. Course coordinator: Cecilia Emanuelsson, Cecilia.Emanuelsson@biochemistry.lu.se, Kemiska institutionen. Prerequisites: TEK285 Chemistry from General Chemistry to the Molecules of Life, TEK295 Biology of the Cell, and TEK015 Human Physiology. The number of participants is limited to 20 Selection criteria: Credits remaining for the degree. Assessment: Written and oral examination. Home page: http://www.mps.lu.se/.

Knowledge and understanding
For a passing grade the student must

have a fundamental understanding of how protein structure is determined, and how the structure in turn determines the function of a protein.
have a fundamental understanding of how enzymes catalyze reactions essential for life.
have a fundamental understanding of how these reactions are regulated.
have a fundamental understanding of common catalytical mechanisms.
have a fundamental understanding of enzyme kinetics, and how these are used to study enzymes.
have a fundamental understanding of the metabolism of the cell, including glyconeogenesis, glycogen metabolism, fatty-acid metabolism, and the metabolism of proteins and amino acids.
have a fundamental understanding of the drug development process.
have a fundamental understanding of the theory behind protein purification and investigation methods, such as electrophoresis, chromatography, centrifuging, spectroscopy, and X-ray crystallography.
have a fundamental understanding of bioinformatics and its applications.

Skills and abilities
For a passing grade the student must

have achieved skills in practical biochemistry, including protein purification, affinity chromatography, gel electrophoresis, and activity measurements with fluorimetry.
have achieved skills in experiment planning.
have achieved skills in analyzing biochemical data and performing enzyme kinetic calculations.
have achieved skills in searching protein databases and literature databases for bioinformatic information, and in analyzing this information with PDB viewer.
have achieved skills in conducting a biochemical discussion at a high intellectual level.

Contents
The structure of the cell; structure and function of nucleic acids, proteins and membranes; enzyme catalysis; electron transport and oxidative phosphorylation; photosynthesis; cellular metabolism and its regulation; cell biological methods.

Literature
Berg, Tymoczko and Stryer: Biochemistry. WH Freeman and Company 2007. ISBN: 0-7167-8724-5.
Precise reading instructions will be available.