Syllabus academic year 2009/2010
(Created 2009-08-11.)

Higher education credits: 7,5. Grading scale: TH. Level: A (Second level). Language of instruction: The course will be given in English on demand. Alternative for: MBIO1, MLIV1. Optional for: B4l, B4mb, K4l. Course coordinator: Senior lecturer Margareta Sandahl, och Tech. D. Clas Wesén 0703-978762,, Teknisk analytisk kemi. Prerequisites: KAK016 Analytical Chemistry (Basic chemistry including fundamental instrumental analytical chemistry and basic chromatography). The course might be cancelled if the numer of applicants is less than 6. The number of participants is limited to 24 Selection criteria: Credits remaining for the degree. Assessment: Written examination covering lectures, course literature and the experimental parts of the course. To receive a passing grade for the course, students must have also satisfactorily completed the required laboratory work and reports. Home page:

The aim of the course is to provide the students with advanced knowledge and insight in analytical chemistry with emphasis on analytical separation techniques. The course also contributes to the development of the ability of the students to independently choose, optimise and perform chemical analyses. The course thus aims to provide the conditions needed for the student to be able to assimilate a coherent overview of the subject, which will underpin his or her ability to create new techniques/methods that will be applicable in new topics arising in the industry.

Knowledge and understanding
For a passing grade the student must

Skills and abilities
For a passing grade the student must

Judgement and approach
For a passing grade the student must

The course considers different analytical techniques and methods for the separation of analytes in gases and liquids for quantitative and qualitative analytical analysis.

Lectures: Advanced theoretical aspects in chromatographic separations including partition and adsorption equilibrium. Instrumentation and experimental techniques for high performance liquid chromatography and capillary gas chromatography with selective detection systems. Coupling of gas chromatography and mass spectrometry. Introduction to capillary electrophoresis and sample preparation and quality assurance.

Experimental work: Quantitative gas chromatography and liquid chromatography and qualitative gas chromatography – mass spectrometry experiments are carried out. These experiments represent common analytical methods that are employed within the food and pharmaceutical industries as well as in environmental analytical chemistry. Examples of applications are determination of theophyllin in human plasma and ascorbic acid in urine with liquid chromatography, and chiral separations of terbutaline enantiomers with capillary electrophoresis.

Harris, D C: Quantitative Chemical Analysis. Seventh ed. Freeman 2007. ISBN: 0-7167-7041-5.
Compendia. Div. of Technical Analytical Chemistry.
Laboratory text. Div. of Technical Analytical Chemistry.