Syllabus academic year 2008/2009
(Created 2008-07-17.)
GENE TECHNOLOGYKBK041

Higher education credits: 7,5. Grading scale: TH. Level: G2 (First level). Language of instruction: The course might be given in English. Compulsory for: B3. Alternative for: MBIO1. Course coordinator: Professor Leif Bülow, leif.bulow@tbiokem.lth.se och Docent Marie Francoise Gorwa-Grauslund, Marie-Francoise.Gorwa@tmb.lth.se, Tillämpad biokemi. Prerequisites: KMB060 Mikrobiologi och KBK011 Biokemi. Assessment: Approved practicals, oral presentation of a literature task and written exam. Parts: 2. Further information: The course is given in collaboration between the divisions of Pure and Applied Biochemistry and Technical Microbiology. Home page: http://www.tbiokem.lth.se.

Aim
The aim of the course is to give the student the ability to understand and critically examine the possibilities and limitations in modern gene technology.

Knowledge and understanding
For a passing grade the student must

Skills and abilities
For a passing grade the student must

Judgement and approach
For a passing grade the student must

Contents
The course deals with the genetic and physiological prerequisites for gene modification of bacterial, yeast, plant and animal cells. Applications in chemical, food and pharmaceutical industries are emphasised. The laboratory part of the course includes the most frequently used methods in gene technology embracing isolation and mapping of DNA, PCR, vector construct, transformation and hybridisation.

Literature
Brown, T.A.: Gene Cloning and DNA Analysis. Blackwell Publishing 2006. ISBN: 1405111216.
Laboratory compendium

Parts

Code: 0105. Name: Gene Technology, Theory.
Higher education credits: 6. Grading scale: TH. Assessment: Written examination. Contents: The course deals with the genetic and physiological prerequisites for gene modification of bacterial, yeast, plant and animal cells. Applications in chemical, food and pharmaceutical industries are emphasised.

Code: 0205. Name: Gene Technology, Practicals.
Higher education credits: 1,5. Grading scale: UG. Assessment: Approved practicals. Contents: The laboratory part of the course includes the most frequently used methods in gene technology embracing isolation and mapping of DNA, PCR, vector construct, transformation and hybridisation.