Course syllabus
Protein Engineering
Protein Engineering
KBK050, 7,5 credits, A (Second Cycle)
Valid for: 2016/17
Decided by: Education Board C
Date of Decision: 2016-04-12
General Information
Main field: Biotechnology.
Elective Compulsory for: MBIO2
Elective for: B4-mb, B4-pt
Language of instruction: The course will be given in English on demand
Aim
The aim of the course is to give a deeper understanding of
protein structure and function.
Learning outcomes
Knowledge and understanding
For a passing grade the student must
- be able to describe, formulate and evaluate methods for
mutations of proteins
- be able to describe and evaluate information about protein
structures on primary, secondary and tertiary levels.
- be able to describe technical and medical applications of
mutated proteins
Competences and skills
For a passing grade the student must
- be able to prepare a site-directed mutation of a protein.
- be able to use software for structure modelling at a low level
of complexity.
- in oral and written form be able to govern and utilise commonly
used forms of nomenclature in protein engineering.
Judgement and approach
For a passing grade the student must
- be able to analyse and critically examine scientific reports
and articles in protein engineering.
- in a group be able to design a modification of a protein in
order to achieve a desired chemical or physical property.
Contents
Random and site-directed mutations of proteins using genetic
methods are described. Combinatorial methods using biological and
chemical approaches are treated. The course also includes a
practical task where the student independently design a modified
protein.
Examination details
Grading scale: TH
Assessment: Written and problem-oriented home exam. Oral group presentation of material taken from the scientific literature. Approved reports from exercises and practicals are also required.
Admission
Admission requirements:
The number of participants is limited to: No
Reading list
- Brown, T.A.: Gene Cloning and DNA Analysis, An Introduction. Blackwell Publishing , 2016, ISBN: 978-1-119-07256-0.
- Brändén, C-I., Tooze, J.: Introduction to Protein Structure. Garland Publishing Inc, 1999, ISBN: 0-8153-2305-0.
- Laboratory compendium, distributed during the course.
- Computer exercise compendium, distributed during the course.
Contact and other information
Course coordinator: Professor Leif Bülow, Leif.Bulow@tbiokem.lth.se
Course homepage: http://www.tbiokem.lth.se