Course syllabus

Genteknik
Gene Technology

KBKF01, 7,5 credits, G2 (First Cycle)

Valid for: 2017/18
Decided by: PLED B/K
Date of Decision: 2017-03-29

General Information

Main field: Biotechnology.
Main field: Technology.
Compulsory for: B3
Elective Compulsory for: MBIO1
Language of instruction: The course will be given in English

Aim

The aim of the course is to give the student the ability to understand and critically examine gene technologies.

Learning outcomes

Knowledge and understanding
For a passing grade the student must

• be able to describe and formulate a simpler gene cloning experiment
• be able to describe, formulate and evaluate different host cells for expression of a foreign protein
• be able to describe applications of gene technology in biotechnology, food technology and medicine.

Competences and skills
For a passing grade the student must

• practically be able to explore the most commonly used techniques in the laboratory.
• be able to follow changes caused by a gene modification.
• be able to describe and evaluate information in scientific articles in gene technology.

Judgement and approach
For a passing grade the student must

• in a group be able to discuss and critically examine scientific literature.
• both in oral and written forms be able to utilise and govern the most commonly used methods and techniques in gene technology.

Contents

The course addresses gene modification of bacterial, yeast, plant and animal cells. Applications in chemical, food and pharmaceutical industries are emphasised. The laboratory part of the course includes the most frequently used methods in gene technology embracing isolation and mapping of DNA, PCR, vector construct, transformation and hybridisation.

Examination details

Grading scale: TH - (U,3,4,5) - (Fail, Three, Four, Five)
Assessment: Approved practicals, oral presentation of a literature task and written exam.

The examiner, in consultation with Disability Support Services, may deviate from the regular form of examination in order to provide a permanently disabled student with a form of examination equivalent to that of a student without a disability.

Parts
Code: 0117. Name: Gene Technology, Theory.
Credits: 6. Grading scale: TH. Assessment: Written examination. Contents: The course deals with the genetic and physiological prerequisites for gene modification of bacterial, yeast, plant and animal cells. Applications in chemical, food and pharmaceutical industries are emphasised.
Code: 0217. Name: Gene Technology, Practicals.
Credits: 1,5. Grading scale: UG. Assessment: Approved practicals. Contents: The laboratory part of the course includes the most frequently used methods in gene technology embracing isolation and mapping of DNA, PCR, vector construct, transformation and hybridisation.

Admission

Admission requirements:

• KMB060 Microbiology or KMBA01 Microbiology
• KBK011 Biochemistry or KBKA10 Biochemistry

The number of participants is limited to: No
The course overlaps following course/s: KBK041

Reading list

• course leaders: Laboratory Compendium. 2013. Laboratory manual.
• T.A. Brown: Gene Cloning and DNA Analysis, An Introduction. Wiley-Blackwell, 2016, ISBN: 978-1-119-07256-0.

Contact and other information

Course coordinator: Dr. Catherine Paul, catherine.paul@tmb.lth.se
Course coordinator: Dr. Magnus Carlquist, magnus.carlquist@tmb.lth.se
Course coordinator: Dr Nelida Leiva Eriksson, Nelida.Leiva_Eriksson@tbiokem.lth.se
Course homepage: http://www.tbiokem.lth.se
Further information: The course is given in collaboration between the divisions of Pure and Applied Biochemistry and Applied Microbiology.